Estrogenic and mutagenic activities of Crotalaria pallida measured by recombinant yeast assay and Ames test

Estrogenic and mutagenic activities of Crotalaria pallida measured by recombinant yeast assay and Ames test

BACKGROUND Crotalaria pallida Ailton is a plant belonging to the Fabaceae family, popularly known as "rattle or rattlesnake" and used in traditional medicine to treat swelling of the joints and as a vermifuge. Previous pharmacological studies have also reported anti-inflammatory, antimicrobial and antifungal activities. Nevertheless, scientific information regarding this species is scarce, and ...

Evaluation of Mutagenic Potentials of Some Food Additives by Ames Test

Some chemicals used by human such as food additives are mutagenic and mutagenicity effects analysis of them is very important because of considerable consumption by man. One of the most common methods used in the recognition of the chemicals' mutagenicity is Salmonella Typhimurium Reverse Mutation (Ames) test. The purpose of the present study is mutagenicity effects analysis of sodium nitrite, ...

Antitumoral, mutagenic and (anti)estrogenic activities of tingenone and pristimerin

Cancer constitutes the second main mortality cause in the world, after cardiovascular diseases. In spite of the progresses in the chemotherapeutics treatments, many patients fail chemotherapy, mainly because of side effects or multidrugs resistance, proving the need and importance of the research for new molecules with anticancer activity, more effective and with smaller adverse effects. Variou...

Assessment of mutagenic and genotoxic activity of Caesalpinia sappan L. extract by Ames test and micronucleus assay

Effect of trypsin inhibitor from Crotalaria pallida

A proteinaceous trypsin inhibitor was purified from Crotalaria pallida seeds by ammonium sulfate precipitation, affinity chromatography on immobilized trypsin–Sepharose and TCA precipitation. The trypsin inhibitor, named CpaTI, had Mr of 32.5 kDa as determined by SDS-PAGE and was composed of two subunits with 27.7 and 5.6 kDa linked by disulfide bridges. CpaTI was stable at 50 °C and lost 40% o...